In vitro and in vivo Activity of some Antibiotics against Staphylococcal Biofilm and Planktonic Cells Isolated from Diabetic Foot Infections

نویسندگان

  • A. Abd El-Aziz
  • T. El-Banna
  • A. Abo-Kamar
  • A. Ghazal
  • R. AboZahra
چکیده

The diabetic foot syndrome is clearly one of the most important complications of diabetes and is the most common cause of hospitalization among diabetic patients. Staphylococcus aureus is found to be the commonest pathogen present in diabetic foot infections. The aim of the present study is to determine activities of three kinds of antibiotics against Staphylococcal biofilm and planktonic cultures in vitro, and to indicate the difference in wound healing between staphylococcal planktonic and biofilm stage of colonization in vivo by using diabetic rat models. Biofilm forming staphylococci were identified by using the modified microtiter plate method. And the effect of different concentrations of several antibiotics (including ciprofloxacin, gentamycin and amoxicillin-clavulanic acid) on eight isolates was determined. The result showed that out of 86 Staphylococcal isolates, eight strains were found to be strong biofilm forming. It was found that the preformed biofilm was very difficult to remove with most isolates even with multiples of the MIC and that the biofilm MBC reached 46 times the planktonic MBC in some isolates. This was also noticed in case of the diabetic foot infection of the rat model, as the treatment was more efficient when it started immediately after infection, before the formation of the biofilm, as the bacterial infection was eliminated within 3-4 days, while it could not be completely eliminated when treatment started after the biofilm formation. This was also observed from the rate of healing and confirmed by histological examination. [A. Abd El-Aziz, T. El-Banna, A. Abo-Kamar, A. Ghazal, and R. AboZahra. In vitro and in vivo Activity of some Antibiotics against Staphylococcal Biofilm and Planktonic Cells Isolated from Diabetic Foot Infections. Journal of American Science 2010;6(12):760-770]. (ISSN: 1545-1003). http://www.americanscience.org.

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تاریخ انتشار 2010